Ticks are currently considered to be second only to mosquitoes as vectors of human infectious diseases in the world. Each tick species has preferred environmental conditions and habitat that determine the geographic distribution of the ticks and, consequently, the risk areas for tick-borne diseases. Tick-borne diseases are becoming more frequently diagnosed as the cause of human infections as animal reservoirs and tick vectors have increased in numbers and humans have inhabited areas where reservoir and tick populations are high.
Ticks may become infected and harbor one or more disease-causing agents (e.g. bacteria, viruses, or parasites) and coinfection can also occur, compounding the difficulty in diagnosis and treatment. Co-infections often prove to cause worse symptoms than a single infection alone. In most cases, patients present severe atypical clinical manifestations, wider range of secondary symptoms, and longer recovery. Co-infections can be challenging to diagnose, as clinical features of tick-borne diseases often overlap. Identifying and treating polymicrobial infections are critical as morbidity and mortality increases substantially if there are delays in diagnosis and treatment.
Jim Dunn, PhD, D(ABMM) from Texas Children’s Hospital Laboratory diagnosis of Bordetella infections: rapid, direct detection and differentiation of B. pertussis and B. parapertussis.
Preeti Pancholi, PhD D(ABMM) from The Ohio State University, University Hospital East discusses Rapid detection of Varicella Zoster Virus Central Nervous System Infections.
Regulatory requirements for verification and validation are complex and often cause confusion among clinical laboratorians, especially in regards to quantitative molecular assays. This presentation will discuss verification and validation requirements as defined by the Clinical Laboratory Improvement Amendments (CLIA) and the College of American Pathologists (CAP). Calibrations and standards, including calibration, calibration verification, analytical measurement range (AMR) verification and the importance of quality standards, will also be discussed.
Appropriate treatment of patients with influenza depends on accurate and timely diagnosis, and early diagnosis can reduce the inappropriate use of antibiotics and provide the option of using antiviral therapy. Diagnostic tests available for influenza include viral culture, serology, rapid antigen testing, reverse transcription PCR, immunofluorescence assays, and rapid molecular assays. Molecular assays are increasingly becoming the diagnostic tests of choice.
Dr. Wallace Greene from Penn State College of Medicine, Milton S. Hershey Medical Center discusses converting Pneumocystis jirovecii testing from DFA to molecular.
Dr. Raquel Martinez from Geisinger Medical Laboratories discusses Group B Strep diagnostics and results from the clinical study for Simplexa™ Group B Strep Direct.
Dr. Livia Berlinger earned a PhD in Biology from the University of Zurich. She is a member of the Swiss Scientific Society of Infectious Diseases and has served as the head of Molecular Diagnostics for Bioanalytica AG in Luzern since 2014.
Bioanalytica represents a laboratory network serving public and private Hospitals since 1957 and has a strong presence in Switzerland. Bioanalytica provides consistent high-quality diagnostics at a national level thanks to a unified effort of more than forty clinicians, biologists and pharmacologists. In her role, Dr. Berlinger manages infectious diseases diagnostics to ensure continuous technological advancement, both in microbiology and virology.
In this seminar, Dr. Berlinger will present an overview of the challenges associated with Bordetella infection, with a specific focus on molecular diagnostics.
Maddalena Peghin, MD, PhD: Herpes Viruses Infections: a clinical overview
Dr. David Boutolleau: Molecular diagnosis of viral meningoencephalitis with the DiaSorin Simplexa solution: experience of the French National Reference Center for Herpesviruses
Michelle Tabb, PhD: A new comprehensive solution for molecular detection of herpetic simplex viruses infections
Whooping cough caused by Bordetella infection has resurged in recent years. Dr. Karissa Culbreath will discuss the clinical presentation of Bordetella infection and its public health impact. The prognostic value of B. pertussis and B. parapertussis detection for Bordetella diagnosis will be reexamined.