DiaSorin Molecular Iam AML1-ETO Kit

Iam AML1-ETO Kit

The faster molecular solution for one-step detection of AML1-ETO translocation

Want to know more?

Get in touch
KIT for Oncohematology

Overview

Background

AML1-ETO/t (8;21)(q22;q22)/ RUNX1-RUNX1T1 is a recurrent genetic abnormality in both childhood and adult Acute Myeloid Leukemia (5-10% of all AML and nearly 40% of the AML FAB M2 subtype). Although this translocation is associated with a good prognosis, only 50% of patients are cured and its rapid molecular identification is crucial for risk stratification of patients and therapeutic decisions.

Why to choose it

Molecular identification of AML1-ETO translocations in 15 minutes directly from RNA. Effective screening of AML is now possible.

For use on

Benefits

DiaSorin Molecular Iam AML1-ETO Kit
Ultra Rapid

Identification of positive samples in less than 15 min, starting from extracted RNA.

Results in < 15 minutes
One step,
closed format

The assay works directly on 500 ng RNA with no need for multistep procedures: decreased risk of contamination and errors.

100% specific

No false positive results and absence of primer dimers.

Validated on 610 replicates

The Analytical Specificity has been established on negative cell line RNA extracted by both the Qiagen RNeasy kit and modified Trizol.

Sample type Total No. Replicates % Analytical Specificity
Sample typeCell lines Total No. Replicates400 % Analytical Specificity100%
Sample typeNTC Total No. Replicates210 % Analytical Specificity100%
Highly reliable

Internal endogenous control and quality controls are included to allow validation of results.

Lab specialist examining a phial with a colleague standing in the background
Highly Sensitive

Detection of the AML1-ETO translocation in 10 thousand folds diluted samples in less than 20 minutes.

160 replicates

The test consistently detects the AML1-ETO transcript down to 10-4 dilution (Kasumi-1 in HL60) in less than 20 minutes, both on Qiagen and manual extracted RNA. Dilutions at 10-5 of Kasumi-1 in HL60, have also been revealed with a probability close to 60%.

Extraction method Kasumi-1 into HL60 RNA dilution No. Replicates Mean Tt (min) % Detection
Extraction methodQiagen Rneasy Kit Kasumi-1 into HL60 RNA dilution10³ No. Replicates40 Mean Tt (min)14,37 % Detection 100%
Extraction methodModified TRIzol Kasumi-1 into HL60 RNA dilution10³ No. Replicates40 Mean Tt (min)14,05 % Detection 100%
Extraction methodQiagen Rneasy Kit Kasumi-1 into HL60 RNA dilution104 No. Replicates40 Mean Tt (min)16,67 % Detection 100%
Extraction methodModified TRIzol Kasumi-1 into HL60 RNA dilution104 No. Replicates40 Mean Tt (min)16,94 % Detection 100%
Clinically validated

100% concordance with the traditional RT-PCR (Biomed Protocol*, by Van Dongen et al)
*Leukemia. 1999 Dec;13(12):1901-28.

65 clinical samples

Clinical archived RNA samples, extracted by Qiagen RNeasy kit, modified TRIzol and Maxwell, have been retrospectively tested by Q-LAMP and the results have been compared RT-PCR and found to be full concordant.

Sample status % Agreement with PCR (BIOMED protocol)
Sample statusAML1-ETO positive % Agreement with PCR (BIOMED protocol)100% (N=35)
Sample statusAML1-ETO negative % Agreement with PCR (BIOMED protocol)100% (N=30)
Specialist at work in a lab with a collegue
Robust

Superior robustness to inhibitors, RNA degradation and suboptimal target quantities.

The intrinsic robustness of the enzyme employed in Q-LAMP results in a enhanced robustness versus the classical inhibitor factors of PCR.

Superiority
vs. PCR

Q-LAMP is a very rapid, easy, accurate, convenient and robust method for detection of AML1-ETO translocations

Q-LAMP RT-PCR (Biomed)
SubjectRNA input Q-LAMP500 ng/rx robust down to 25 ng/rx RT-PCR (Biomed)1μg/rx
SubjectSteps Q-LAMP1 RT-PCR (Biomed)3
SubjectAssay format Q-LAMPMULTIPLEX RT-PCR (Biomed)SIMPLEX
SubjectControl of reaction Q-LAMPINTERNAL RT-PCR (Biomed)EXTERNAL
SubjectRobustness Q-LAMP– to chemical contamination
– to PCR inhibitors
– to degraded samples
RT-PCR (Biomed)To chemical contamination
SubjectResult interpretation Q-LAMPOBJECTIVE RT-PCR (Biomed)SUBJECTIVE
SubjectTime to result Q-LAMP40min RT-PCR (Biomed)3h 30min

Libraries


Ordering info

Product Code Reactions
Product

Iam AML1-ETO

Code V35AML Reactions 24

Do you want to place an order or request a trial?

Discover more

Our repository

All the answers you need.
Access our database to find:
instructions for use, safety data sheets,
assay protocols and more.

Access our repository